Analysis of bisphenol A leaching from baby feeding bottles

Bisphenol A can leach out from the plastic surfaces of food containers and has been detected in various matrices such as plasma, urine and groundwater. Bipshenol A is an endocrine disruptor, which can mimic the body’s own hormones such as estrogen and may lead to negative health effects. In this Application Note, we describe a method to quantify bisphenol A and a structurally similar analog bisphenol F extracted from baby feeding bottles. The method was developed on an Agilent 1260 Infinity LC system using an Agilent ZORBAX Eclipse Plus C18 column. Partial method validation was performed to demonstrate linearity, robustness and precision of area and retention time. The dilution series was generated automatically using an Agilent 7696A Sample Prep WorkBench, saving analyst time. The limit of quantitation (LOQ) for bisphenol A was found to be 1.06 ng/mL. During sample recovery studies, 80% recovery was obtained for bisphenol A. The method was transferred to an ultrahigh performance liquid chromatography (UHPLC) method using an Agilent 1290 Infinity LC System. The UHPLC method has the same experimental conditions but showed narrower and higher peaks, better resolution, and improved signal-to-noise response. Both methods can be applied for bisphenol A quantification in quality control of food containers such as baby feeding bottles. Author Syed Salman Lateef Agilent Technologies, Inc. Bangalore, India